to set up the software for your use. This guide shows a workflow that uses application settings. BD FACSDiva Software Quick Reference Guide for the BD LSR II. Compensation Controls in the BD FACSDiva Software Reference Manual. • Default templates are now provided for certain instrument functions. If you are the first user of the day: Boot up the computer and log on to Windows. Wait 10 seconds, then Open the BD FACSDiva software and log on. Allow the.

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Product name, part number, and serial number; software version and computer system specifications Error messages, if any Details of recent cytometer performance BD Biosciences might also request the console. To move objects between worksheets, use the copy and paste functions; objects cannot be dragged.

For example, the Inspector can be used to change plot attributes like the background color, title, axes labels, and scale, or to enter the name of an experiment, specimen, or tube. The starting value the lowest level is half the value entered. When recording is complete and acquisition is stopped, statistics are calculated on the faczdiva number of recorded events. Whether viewed on one monitor or two, workspace windows can be resized and repositioned for the most efficient operator workflow.

Complying with all applicable copyright laws is More information.

Copy and paste wells to blank positions in the Plate window. See Adding New Elements to the Browser on page Additional preferences might be available for your fxcsdiva type; refer to your instrument manual for a description.

A maximum of 50 global worksheets can be set up for each experiment, and each global worksheet can contain up to 10 pages. After you move or resize a snap-to gate, the gate no longer adjusts automatically. The plot expands to twice its size. This material briefly explains how information is stored in Windows More information.

Bd Facsdiva Software

NOTE If a workstation does not have a D drive, then the default locations for output files will be created on the C drive. To start the software: If you set the default background to black, select the checkbox mxnual print plots with a white background; white gates and populations are then automatically printed in black. Comments can be viewed when you are importing a panel template. Video display images copyright More information.

Instrument Name During software installation, each instrument is assigned a name and serial number. This section describes how to subset populations using the population hierarchy. Only one object can be copied and pasted at a time.

BD FACSCANTO II QUICK REFERENCE MANUAL Pdf Download.

If a worksheet with the name of siftware specimen and tube already exists, the copied elements are pasted into the existing worksheet. As you change fluorochromes, filters, or mirrors, you can softsare between mahual configurations that match your instrument optics.

Locate and read topics specific to the operation you are performing. For example, experiment templates can be assigned to the New Experiment button, panel templates can be assigned to the New Specimen button, and analysis templates can be assigned to the New Tube or New Global Worksheet button. This section describes how to export experiments and delete them after export; deleting experiments is described on page Select this checkbox to always update the cytometer settings to the latest performance check.

Close the region by double-clicking or by clicking on the first vertex. No part of this publication may be reproduced, transmitted, transcribed, stored. The image is stored in memory until it is pasted into another application.

Start display at page:. All tubes must be recorded with consistent PMT voltages.

BD FACSCanto II Quick Reference Manual

Facsdiva Manual

Statistics are displayed in statistics views and the values are updated in accordance with the Events to Display setting. Edit this number by selecting one or more fields and then entering a new number.

To move a window, drag the title bar to a new position on the screen. Cytometer settings can be applied at the experiment, specimen, or tube level. The computer is left on at all times. Otherwise, generic value is applied. Provides an interface for setting up experiments. Laser Controls Lasers are instrument-specific, thus laser controls for your instrument might be different from those shown in this section. The chosen worksheet is displayed automatically when you select a tube below this specimen.

The following tutorials describe how to add an administrator password and create a new user, and show how workspace setup, user preferences, and the Browser view differ from one user to another. A summary of menus is provided in Shortcut Menus on page Using Gating Features Acquisition Setup Use Acquisition Setup fields to control the number of events to record and display.

BD Biosciences welcomes customer input on corrections and suggestions for improvement. Global worksheets are displayed in this folder in the order they were created.

BD FACSDiva Software Reference Manual

Interval Gate button—allows a range of events to be selected on a dot, contour, or histogram plot. If needed, start your cytometer and log into the software before proceeding.

Click the intersection of the quadrant markers.

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BD Biosciences brand of digital flow cytometers, including the FACSCanto, the LSR-II, FACSAria and Fortessa, utilize a software acquisition program known as FACSDiva.

Diva is aptly named as it can be a difficult program to master.

However, Diva has come along way over the past 10 years and many improvements have been made to help end-user.

Taking time to learn these changes will improve the reproducibility of your data, the chances of your data getting published, and your overall experience on the cytometer.

These changes will also save you time. Here are 8 time-saving FACSDiva tips to use the during your next flow experiment:

1. Compensation doesn’t require the ‘universal negative.’

It is important to remember that the background of the positive and negatives should be matched. The use of a universal negative will cause problems in compensation, especially if cells and beads are used for generating the compensation matrix.

In Diva, you can unselect the ‘use a separate tube for negative control’ when you’re setting up compensation. This will allocate the placement of a P3 gate on the single-stained histograms. Diva will then use the P3 gate as the negative population when calculating compensation.

2. Make multiple matrices.

In the case where several different antigens are being measured in the same channel, especially where the label is a tandem dye, it is critical to have a different compensation matrix for each tandem combination.

In Diva, when you create a compensation matrix, you can add additional columns of the channel in question, and label each one with the appropriate antigen name. Then, after collecting the controls and performing compensation, when you are finally collecting your samples, make sure each one is labeled w